phusion dna

  • Taq Thermo Scientific Phusion DNA Polymerases

    Phusion DNA Polymerases are an ideal choice for amplification of long templates The high enzyme processivity allows amplification of a wide variety of template sizes Amplicons up to 20 kb are produced with high yields short cycling times and high fidelity Fast PCR with high fidelity Phusion DNA Polymerases incorporate a high number of

  • PhusionDNA Thermo Fisher

    Phusion Green High Fidelity DNA Polymerase 2 U/µL 100 U ¥770 ¥539 F534L Phusion Green High Fidelity DNA Polymerase 2 U/µL 500 U ¥3 058 ¥2 141 F531S Phusion High Fidelity PCR Master Mix with HF Buffer 100 x 50 μL rxns ¥1 243 ¥870 F531L

  • Thermo Scientific Phusion High Fidelity DNA Polymerase

    The Phusion DNA Polymerase generates long templates with an accuracy and speed previously unattainable with a single enzyme even on the most difficult templates The extreme fidelity makes Phusion DNA Polymerase a superior choice for cloning Using a lacI based method modified from previous studies1 the error rate of Phusion DNA Polymerase in

  • Phusion High Fidelity DNA Polymerase from Finnzymes Oy

    The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a

  • Phusion Site Directed Mutagenesis Kit User Guide

    The Phusion DNA polymerase ensures high fidelity for the exponential amplification thus reducing unwanted secondary mutations and enabling amplification of large plasmids up to 10 kb A hot start modification in the Phusion DNA polymerase combines the DNA polymerase

  • PhusionOpenWetWare

    add to 50μl Phusion DNA polymerase 2U/μ 0 5μl 0 02 U/pl A 2x supermix is now available containing either HF buffer or GC buffer dNTPs and Phusion polymerase See references section below for links

  • Thermo Scientific Phusion High Fidelity DNA Polymerase

    Phusion DNA Polymerase should be pipetted carefully and gently as the high glycerol content 50 in the storage buffer may otherwise lead to pipetting errors It is critical that the Phusion DNA Polymerase is the last component added to the PCR mixture since the enzyme exhibits 3´→5´ absence of dNTPs Due to the nature of Phusion DNA

  • Phusion High Fidelity DNA Polymerase 2 U/µL

    Thermo Scientific Phusion High Fidelity DNA Polymerases set a gold standard for high performance PCR Featuring an error rate 50 fold lower than that of Taq and 6 fold lower than that of Pfu Phusion High Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity Phusion DNA Polymerases offer robust performance with short protocol times even in the presence

  • Guidelines for PCR Optimization with Phusion High Fidelity

    The Phusion DNA Polymerase has the ability to stablize primer template hybridization As a basic rule for primers >20 nt anneal for 10 30 seconds at Tm 3 C of the lower Tm primer The Tm s should be calculated with the nearest neighbor method as results from primer Tm calculations can vary significantly depending on the method used

  • Phusion Plus DNA Polymerase/Phusion Plus

    Thermo Scientific Phusion Plus DNA Polymerase is a hot start high fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing It is a Pyrococcus like proofreading DNA polymerase fused to a processivity enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance

  • PCR amplification of plasmid DNA Phusion Benchling

    PCR amplification of plasmid DNA Phusion Updated 3/8/2019 7 43pm Step by step by SoundBio iGEM created by Yoshi Goto Introduction This protocol describes the method used for the amplification of specific DNA fragments by the Polymerase Chain Reaction using plasmid DNA as a template The amplified products may subsequently be analysed

  • Frontiers Insertion of Badnaviral DNA in the Late Blight

    PCRs were done using Phusion DNA polymerase NEB Ipswich MA United States PCR mixes with a final volume of 25 μl contained 2 5 μl of 10 GC buffer 0 2 μl of 25 mM MgCl 2 1 6 μl of 2 5 mM dNTPs 1 U of 5 000 U/ml Phusion DNA polymerase and 5 μl of each primer at a concentration of 5 μM Thermocycling conditions were an initial

  • Phusion DNA PolymeraseNew England Biolabs GmbH

    Phusion Technology Phusion DNA Polymerase brings together a novel Pyrococcus like enzyme with a processivity enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates Additionally Phusion DNA Polymerase is capable of amplifying long templates In the quality control tests of Phusion DNA

  • PCR Reaction PCR Conditions For Phusion DNA

    For Phusion DNA Polymerase Thermo Fisher Standard 5X Phusion HF buffer 10 ul 98 C1 min 10mM dNTP1 ul 98 C15 s Primers/each 10µM 2 5 ul 55 C20 s 30 cycles cDNA OR0 5 ul 72 C Genomic DNA OR0 5 ul 72 C4 min Plasmid DNA1 0 ul 4 Cfor ever

  • Phusion High Fidelity DNA Polymerases Products NEB

    Phusion High Fidelity PCR Kit Based on your Freezer Program type you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart Please review and update your order accordingly If you have any questions please contact Customer Service at freezers neb or x 8

  • Phusion DNA PolymeraseNew England Biolabs

    Phusion DNA Polymerase was developed by Finnzymes Oy now a part of Thermo Fisher Scientific This product is manufactured by New England Biolabs Inc under agreement with and under the performance specifications of Thermo Fisher Scientific Phusion is a registered trademark and property of Thermo Fisher Scientific

  • Thermo Scientific Phusion Green High Fidelity DNA

    Phusion DNA Polymerase per 50 μL reaction volume gives good results but the optimal amount can range from 0 5 to 2 units per 50 μL reaction depending on amplicon length and difficulty It is not recommended to exceed 2 U/50 μL 0 04 U/μL especially for amplicons that are > 5kb

  • LabAid Phusion High Fidelity DNA Polymerases

    Phusion DNA polymerase 0 5 μL 0 2 μL 0 02 U/μL Water To 50 μL total To 20 μL total If you are using any of the Phusion PCR master mix products add 25 or 10 μL of the 2X master mix depending on the final reaction volume

  • Phusion Plus Green PCR Master Mix/Phusion

    Phusion Plus DNA Polymerase is a hot start high fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing It is a Pyrococcus like proofreading DNA polymerase fused to a processivity enhancing domain enabling Phusion Plus Green PCR Master Mix to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance

  • Phusion Plus DNA PolymeraseThermo Fisher Scientific

    Phusion Plus DNA Polymerase 50 µL 250 µL 4 250 µL –25 C to –15 C Phusion Plus Buffer 1 25 mL 5 1 25 mL 20 1 25 mL Phusion GC Enhancer 1 25 mL 4 1 25 mL 16 1 25 mL General guidelines Use 98 C for denaturation Use 15–30 s/kb for extension

  • Phusion Plus DNA Polymerase

    Thermo Scientific Phusion Plus DNA Polymerase is a hot start high fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing It is a Pyrococcus like proofreading DNA polymerase fused to a processivity enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and inhibitor tolerance

  • Phusion High Fidelity DNA PolymeraseSevier Lab

    Phusion DNA Polymerase in Phusion HF Buffer is determined to be 4 4 x 10 7 which is approximately 50 fold lower than that of Thermus aquaticus DNA polymerase and 6 fold lower than that of Pyrococcus furiosus DNA polymerase Phusion DNA Polymerase possesses the following activities 5´→3´ DNA polymerase activity and 3´→5´ exonuclease

  • Phusion High Fidelity DNA Polymerases Flyer

    unique fusion technique Phusion DNA polymerases generate PCR products with very high accuracy and speed In addition Phusion DNA polymerases are tolerant of various inhibitors allowing for robust amplifi cation with minimal optimization The processivity of Phusion DNA polymerases is 10 fold greater than that of Pfu DNA polymerase and twice

  • Phusion High Fidelity DNA Polymerase

    Phusion DNA polymerase gave strong specific bands even with the shortest extension time completing the 3 8 kb fragment with only a one minute combined annealing and extension step Enzyme amounts also indicate that significantly less of the highly processive Phusion DNA polymerase is required to complete the task

  • Phusion High Fidelity DNA Polymerase NEB

    Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons With an error rate > 50 fold lower than that of Taq DNA Polymerase and 6 fold lower than that of Pyrococcus furiosus DNA Polymerase 1 Phusion is one

  • DNA Polymerase Selection Chart NEB

    DNA Polymerase Selection Chart The following table lists properties that should be considered when choosing a polymerase Since these properties can depend on reaction conditions the primary references should be consulted prior to use in a given application PCR

  • Phusion high fidelity DNA polymerase

    Phusion high fidelity DNA polymerase Phusion high fidelity DNA polymerase 100U 350 00 500U 1 200 00 1000U

  • Phusion High Fidelity DNA Polymerases NEB

    DNA polymerases with high fidelity are important for applications in which the DNA sequence needs to be correct after amplification Manufactured and quality controlled at New England Biolabs Thermo Scientific Phusion High Fidelity DNA Polymerase offers both high fidelity and robust performance and thus can be used for all PCR applications Its unique structure a novel Pyrococcus like

  • Thermo Scientific Phusion High–Fidelity DNA Polymerase

    The Phusion High–Fidelity DNA Polymerase should be pipetted carefully and gently as the high glycerol content 50 in the storage buffer may otherwise lead to pipetting errors Due to the nature of the Phusion High–Fidelity DNA Polymerase the optimal reaction conditions may differ from PCR protocols for standard DNA

  • Phusion High Fidelity PCR Kit NEB

    Phusion DNA Polymerase was developed by Finnzymes Oy now a part of Thermo Fisher Scientific This product is manufactured by New England Biolabs Inc under agreement with and under the performance specifications of Thermo Fisher Scientific Phusion is a registered trademark and property of Thermo Fisher Scientific

  • PCR Protocol Phusion DNA Polymerase NEB

    Phusion DNA Polymerase may be diluted in 1X HF or GC Buffer just prior to use in order to reduce pipetting errors Please note that protocols with Phusion DNA Polymerase may differ from protocols with other standard polymerases As such conditions recommended below

  • Phusion Plus DNA Polymerase/Phusion Plus

    2 days ago Thermo Scientific Phusion Plus DNA Polymerase is a hot start high fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing It is a Pyrococcus like proofreading DNA polymerase fused to a processivity enhancing domain enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy sensitivity and

  • Phusion High Fidelity DNA Polymerase New England

    Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´ exonuclease activity and will generate blunt ended products Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer as well as 5X Phusion GC Buffer which can be used for complex or GC rich templates